Gibberellin-associated cisgenes modify growth, stature and wood properties in Populus.
Identifieur interne : 002E56 ( Main/Exploration ); précédent : 002E55; suivant : 002E57Gibberellin-associated cisgenes modify growth, stature and wood properties in Populus.
Auteurs : Katherine M. Han [États-Unis] ; Palitha Dharmawardhana ; Reneé S. Arias ; Cathleen Ma ; Victor Busov ; Steven H. StraussSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2011.
Descripteurs français
- KwdFr :
- Analyse en composantes principales (MeSH), Bois (anatomie et histologie), Bois (croissance et développement), Bois (génétique), Données de séquences moléculaires (MeSH), Gibbérellines (métabolisme), Gènes de plante (physiologie), Génie génétique (MeSH), Modèles linéaires (MeSH), Phénotype (MeSH), Populus (anatomie et histologie), Populus (croissance et développement), Populus (génétique), Protéines végétales (génétique), Protéines végétales (physiologie), Régénération (génétique), Transduction du signal (MeSH).
- MESH :
- anatomie et histologie : Bois, Populus.
- croissance et développement : Bois, Populus.
- génétique : Bois, Populus, Protéines végétales, Régénération.
- métabolisme : Gibbérellines.
- physiologie : Gènes de plante, Protéines végétales.
- Analyse en composantes principales, Données de séquences moléculaires, Génie génétique, Modèles linéaires, Phénotype, Transduction du signal.
English descriptors
- KwdEn :
- Genes, Plant (physiology), Genetic Engineering (MeSH), Gibberellins (metabolism), Linear Models (MeSH), Molecular Sequence Data (MeSH), Phenotype (MeSH), Plant Proteins (genetics), Plant Proteins (physiology), Populus (anatomy & histology), Populus (genetics), Populus (growth & development), Principal Component Analysis (MeSH), Regeneration (genetics), Signal Transduction (MeSH), Wood (anatomy & histology), Wood (genetics), Wood (growth & development).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Gibberellins.
- anatomy & histology : Populus, Wood.
- genetics : Populus, Regeneration, Wood.
- growth & development : Populus, Wood.
- physiology : Genes, Plant, Plant Proteins.
- Genetic Engineering, Linear Models, Molecular Sequence Data, Phenotype, Principal Component Analysis, Signal Transduction.
Abstract
We studied the effects on plant growth from insertion of five cisgenes that encode proteins involved in gibberellin metabolism or signalling. Intact genomic copies of PtGA20ox7, PtGA2ox2,Pt RGL1_1, PtRGL1_2 and PtGAI1 genes from the genome-sequenced Populus trichocarpa clone Nisqually-1 were transformed into Populus tremula × alba (clone INRA 717-1B4), and growth, morphology and xylem cell size characterized in the greenhouse. Each cisgene encompassed 1-2 kb of 5' and 1 kb of 3' flanking DNA, as well as all native exons and introns. Large numbers of independent insertion events per cisgene (19-38), including empty vector controls, were studied. Three of the cisgenic modifications had significant effects on plant growth rate, morphology or wood properties. The PtGA20ox7 cisgene increased rate of shoot regeneration in vitro, accelerated early growth, and variation in growth rate was correlated with PtGA20ox7 gene expression. PtRGL1_1 and PtGA2ox2 caused reduced growth, while PtRGL1_2 gave rise to plants that grew normally but had significantly longer xylem fibres. RT-PCR studies suggested that the lack of growth inhibition observed in PtRGL1_2 cisgenic plants was a result of co-suppression. PtGAI1 slowed regeneration rate and both PtGAI1 and PtGA20ox7 gave rise to increased variance among events for early diameter and volume index, respectively. Our work suggests that cisgenic insertion of additional copies of native genes involved in growth regulation may provide tools to help modify plant architecture, expand the genetic variance in plant architecture available to breeders and accelerate transfer of alleles between difficult-to-cross species.
DOI: 10.1111/j.1467-7652.2010.00537.x
PubMed: 20573046
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Arias</name></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name></author><author><name sortKey="Busov, Victor" sort="Busov, Victor" uniqKey="Busov V" first="Victor" last="Busov">Victor Busov</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20573046</idno><idno type="pmid">20573046</idno><idno type="doi">10.1111/j.1467-7652.2010.00537.x</idno><idno type="wicri:Area/Main/Corpus">003144</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003144</idno><idno type="wicri:Area/Main/Curation">003144</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003144</idno><idno type="wicri:Area/Main/Exploration">003144</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Gibberellin-associated cisgenes modify growth, stature and wood properties in Populus.</title><author><name sortKey="Han, Katherine M" sort="Han, Katherine M" uniqKey="Han K" first="Katherine M" last="Han">Katherine M. Han</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Dharmawardhana, Palitha" sort="Dharmawardhana, Palitha" uniqKey="Dharmawardhana P" first="Palitha" last="Dharmawardhana">Palitha Dharmawardhana</name></author><author><name sortKey="Arias, Renee S" sort="Arias, Renee S" uniqKey="Arias R" first="Reneé S" last="Arias">Reneé S. Arias</name></author><author><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name></author><author><name sortKey="Busov, Victor" sort="Busov, Victor" uniqKey="Busov V" first="Victor" last="Busov">Victor Busov</name></author><author><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Genes, Plant (physiology)</term><term>Genetic Engineering (MeSH)</term><term>Gibberellins (metabolism)</term><term>Linear Models (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phenotype (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (physiology)</term><term>Populus (anatomy & histology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Principal Component Analysis (MeSH)</term><term>Regeneration (genetics)</term><term>Signal Transduction (MeSH)</term><term>Wood (anatomy & histology)</term><term>Wood (genetics)</term><term>Wood (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse en composantes principales (MeSH)</term><term>Bois (anatomie et histologie)</term><term>Bois (croissance et développement)</term><term>Bois (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Gibbérellines (métabolisme)</term><term>Gènes de plante (physiologie)</term><term>Génie génétique (MeSH)</term><term>Modèles linéaires (MeSH)</term><term>Phénotype (MeSH)</term><term>Populus (anatomie et histologie)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (physiologie)</term><term>Régénération (génétique)</term><term>Transduction du signal (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Gibberellins</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Bois</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Bois</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Regeneration</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bois</term><term>Populus</term><term>Protéines végétales</term><term>Régénération</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Gibbérellines</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Gènes de plante</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Genes, Plant</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Engineering</term><term>Linear Models</term><term>Molecular Sequence Data</term><term>Phenotype</term><term>Principal Component Analysis</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse en composantes principales</term><term>Données de séquences moléculaires</term><term>Génie génétique</term><term>Modèles linéaires</term><term>Phénotype</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We studied the effects on plant growth from insertion of five cisgenes that encode proteins involved in gibberellin metabolism or signalling. Intact genomic copies of PtGA20ox7, PtGA2ox2,Pt RGL1_1, PtRGL1_2 and PtGAI1 genes from the genome-sequenced Populus trichocarpa clone Nisqually-1 were transformed into Populus tremula × alba (clone INRA 717-1B4), and growth, morphology and xylem cell size characterized in the greenhouse. Each cisgene encompassed 1-2 kb of 5' and 1 kb of 3' flanking DNA, as well as all native exons and introns. Large numbers of independent insertion events per cisgene (19-38), including empty vector controls, were studied. Three of the cisgenic modifications had significant effects on plant growth rate, morphology or wood properties. The PtGA20ox7 cisgene increased rate of shoot regeneration in vitro, accelerated early growth, and variation in growth rate was correlated with PtGA20ox7 gene expression. PtRGL1_1 and PtGA2ox2 caused reduced growth, while PtRGL1_2 gave rise to plants that grew normally but had significantly longer xylem fibres. RT-PCR studies suggested that the lack of growth inhibition observed in PtRGL1_2 cisgenic plants was a result of co-suppression. PtGAI1 slowed regeneration rate and both PtGAI1 and PtGA20ox7 gave rise to increased variance among events for early diameter and volume index, respectively. Our work suggests that cisgenic insertion of additional copies of native genes involved in growth regulation may provide tools to help modify plant architecture, expand the genetic variance in plant architecture available to breeders and accelerate transfer of alleles between difficult-to-cross species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20573046</PMID><DateCompleted><Year>2011</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2011</Year><Month>01</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Gibberellin-associated cisgenes modify growth, stature and wood properties in Populus.</ArticleTitle><Pagination><MedlinePgn>162-78</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1467-7652.2010.00537.x</ELocationID><Abstract><AbstractText>We studied the effects on plant growth from insertion of five cisgenes that encode proteins involved in gibberellin metabolism or signalling. Intact genomic copies of PtGA20ox7, PtGA2ox2,Pt RGL1_1, PtRGL1_2 and PtGAI1 genes from the genome-sequenced Populus trichocarpa clone Nisqually-1 were transformed into Populus tremula × alba (clone INRA 717-1B4), and growth, morphology and xylem cell size characterized in the greenhouse. Each cisgene encompassed 1-2 kb of 5' and 1 kb of 3' flanking DNA, as well as all native exons and introns. Large numbers of independent insertion events per cisgene (19-38), including empty vector controls, were studied. Three of the cisgenic modifications had significant effects on plant growth rate, morphology or wood properties. The PtGA20ox7 cisgene increased rate of shoot regeneration in vitro, accelerated early growth, and variation in growth rate was correlated with PtGA20ox7 gene expression. PtRGL1_1 and PtGA2ox2 caused reduced growth, while PtRGL1_2 gave rise to plants that grew normally but had significantly longer xylem fibres. RT-PCR studies suggested that the lack of growth inhibition observed in PtRGL1_2 cisgenic plants was a result of co-suppression. PtGAI1 slowed regeneration rate and both PtGAI1 and PtGA20ox7 gave rise to increased variance among events for early diameter and volume index, respectively. Our work suggests that cisgenic insertion of additional copies of native genes involved in growth regulation may provide tools to help modify plant architecture, expand the genetic variance in plant architecture available to breeders and accelerate transfer of alleles between difficult-to-cross species.</AbstractText><CopyrightInformation>© 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Katherine M</ForeName><Initials>KM</Initials><AffiliationInfo><Affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dharmawardhana</LastName><ForeName>Palitha</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Arias</LastName><ForeName>Reneé S</ForeName><Initials>RS</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Cathleen</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Busov</LastName><ForeName>Victor</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Strauss</LastName><ForeName>Steven 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J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005875">Gibberellins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005875" MajorTopicYN="N">Gibberellins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016014" MajorTopicYN="N">Linear Models</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025341" MajorTopicYN="N">Principal Component Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012038" MajorTopicYN="N">Regeneration</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20573046</ArticleId><ArticleId IdType="pii">PBI537</ArticleId><ArticleId IdType="doi">10.1111/j.1467-7652.2010.00537.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oregon</li></region></list><tree><noCountry><name sortKey="Arias, Renee S" sort="Arias, Renee S" uniqKey="Arias R" first="Reneé S" last="Arias">Reneé S. Arias</name><name sortKey="Busov, Victor" sort="Busov, Victor" uniqKey="Busov V" first="Victor" last="Busov">Victor Busov</name><name sortKey="Dharmawardhana, Palitha" sort="Dharmawardhana, Palitha" uniqKey="Dharmawardhana P" first="Palitha" last="Dharmawardhana">Palitha Dharmawardhana</name><name sortKey="Ma, Cathleen" sort="Ma, Cathleen" uniqKey="Ma C" first="Cathleen" last="Ma">Cathleen Ma</name><name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name></noCountry><country name="États-Unis"><region name="Oregon"><name sortKey="Han, Katherine M" sort="Han, Katherine M" uniqKey="Han K" first="Katherine M" last="Han">Katherine M. Han</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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